Journal: The ocular surface
Article Title: Mineralocorticoid receptor expression and the effects of the mineralocorticoid receptor antagonist spironolactone in a murine model of graft-versus-host disease.
doi: 10.1016/j.jtos.2024.10.004
Figure Lengend Snippet: Fig. 3. Expression of the mineralocorticoid receptor (NR3C2) in the epithelium of the ocular surface: Immunoblotting analysis of NR3C2 using TKE2 cells (a). Immunohistochemical images including conjunctiva, limbus, and peripheral cornea (b). Sections of limbus (c), central cornea (d) and conjunctiva (e) from syngeneic control mice and cGVHD mice treated with vehicle or spironolactone eyedrops were stained for a mineralocorticoid marker NR3C2 (red) (n = 5–7 per group). Data are presented as the mean ± standard error of the mean. *P < .05, based on the Kruskal–Wallis test with Dunn’s post-hoc test., Scale bar, 50 μm.
Article Snippet: This process was applied to antibodies against nuclear receptor subfamily 3 group C member 2 (NR3C2) (21854-1-AP, Proteintech, Rosemont, IL), allophycocyanin-labeled CD45 (103112, 30-F11, BioLegend, San Diego, CA), heat shock protein 47 (HSP47) (ADI-SPA-470-F, M16.10A1, Enzo Life Science, Farmingdale, NY), and fluorescein isothiocyanate-labeled CD4 (100510, RM-4-5, Biolegend).
Techniques: Expressing, Western Blot, Immunohistochemical staining, Control, Staining, Marker